Marcel Kwiatkowski

Curriculum Vitae

Link to his Curriculum Vitae.

Lab for Bioanalytics & Intermediary Metabolism

Our research aims to elucidate molecular mechanisms at the interface of metabolism and protein function in the context of cellular homeostasis, systems biology, epigenetics, cancer and metabolic diseases. The Kwiatkowski lab specialises in mass spectrometry-based cross-omics. This includes state-of-the-art proteomics, metabolomics and metabolic flux analysis. We combine cross-omics with computational modelling, cell and molecular biology to study the regulatory interplay between cellular metabolism and protein modifications. Our technological advances have always been driven by research questions that cannot be addressed with current tools, from basic biochemistry to personalised medicine and applied biotechnology. We are currently investigating the cellular dynamics of hub metabolites (CoA, acetyl-CoA & acyl-CoAs, SAM, NAD, ATP) and how these molecules control protein modification signatures (acylation, methylation, phosphorylation) and regulatory circuits.

Presentation

Functional tracer proteo-metabolomics: a toolbox to study cellular metabolism and the regulatory interplay between hub molecules and protein modifications.

In our laboratory, we use a combination of proteo-metabolomics, fluxomics, and computational modelling to study cellular metabolism and the regulatory interplay between hub molecules and histone modifications. We have developed a method of multiplexed tracer metabolomics that enables rapid metabolic phenotyping using mass spectrometry.

By combining metabolic flux analysis with proteo-metabolomics, we can simultaneously determine the rates of acetyl-CoA synthesis and histone acetylation dynamics. Without integrating acetyl-CoA and histone acetylation dynamics, metabolic alterations induced by AKT inhibition would be erroneously interpreted as changes in histone acetylation dynamics, rather than as alterations in metabolic flux from glucose to acetyl-CoA.